Developing Porous Fibrin Scaffolds with Tunable Anisotropic Features to Direct Myoblast Orientation.

Functional regeneration of anisotropically aligned tissues such as ligaments, microvascular networks, myocardium, or skeletal muscle requires a temporal and spatial series of biochemical and biophysical cues to direct cell functions that promote native tissue regeneration. When these cues are lost during traumatic injuries such as volumetric muscle loss (VML), scar formation occurs, limiting the regenerative capacity of the tissue. Currently, autologous tissue transfer is the gold standard for treating injuries such as VML but can result in adverse outcomes including graft failure, donor site morbidity, and excessive scarring. Tissue-engineered scaffolds composed of biomaterials, cells, or both have been investigated to promote functional tissue regeneration but are still limited by inadequate tissue ingrowth. These scaffolds should provide precisely tuned topographies and stiffnesses using proregenerative materials to encourage tissue-specific functions such as myoblast orientation, followed by aligned myotube formation and recovery of functional contraction. In this study, we describe the design and characterization of novel porous fibrin scaffolds with anisotropic microarchitectural features that recapitulate the native tissue microenvironment and offer a promising approach for regeneration of aligned tissues. We used directional freeze-casting with varied fibrin concentrations and freezing temperatures to produce scaffolds with tunable degrees of anisotropy and strut widths. Nanoindentation analyses showed that the moduli of our fibrin scaffolds varied as a function of fibrin concentration and were consistent with native skeletal muscle tissue. Quantitative morphometric analyses of myoblast cytoskeletons on scaffold microarchitectures demonstrated enhanced cell alignment as a function of microarchitectural morphology. The ability to precisely control the anisotropic features of fibrin scaffolds promises to provide a powerful tool for directing aligned tissue ingrowth and enhance functional regeneration of tissues such as skeletal muscle.

Nitrogen fertilization enhances organic carbon accumulation in topsoil mainly by improving photosynthetic C assimilation in a salt marsh.

Continuous nitrogen (N) loading alters plant growth and subsequently has the potential to impact soil organic carbon (SOC) accumulation in salt marshes. However, the knowledge gap of photosynthesized carbon (C) allocation in plant-soil-microbial systems hampers the quantification of C fluxes and the clarification of the mechanisms controlling the C budget under N loading in salt marsh ecosystems. To address this, we conducted an N fertilization field observation combined with a 5 h 13C-pulse labeling experiment in a salt marsh dominated by Suaeda. salsa (S. salsa) in the Yellow River Delta (YRD), China. N fertilization increased net 13C assimilation of S. Salsa by 277.97%, which was primarily allocated to aboveground biomass and SOC. However, N fertilization had little effect on 13C allocation to belowground biomass. Correlation analysis showed that 13C incorporation in soil was significantly and linearly correlated with 13C incorporation in shoots rather than in roots both in a 0 N (0 g N m-2 yr-1) and +N (20 g N m-2 yr-1) group. The results suggested that SOC increase under N fertilization was mainly due to an increased C assimilation rate and more efficient downward transfer of photosynthesized C. In addition, N fertilization strongly improved the 13C amounts in the chloroform-labile SOC component by 295.26%. However, the absolute increment of newly fix 13C mainly existed in the form of residual SOC, which had more tendency for burial in the soil. Thus, N fertilization enhanced SOC accumulation although C loss increased via belowground respiration. These results have important implications for predicting the carbon budget under further human-induced N loading.

Reducing retraction in engineered tissues through design of sequential growth factor treatment.

Heart valve disease is associated with high morbidity and mortality worldwide, resulting in hundreds of thousands of heart valve replacements each year. Tissue engineered heart valves (TEHVs) have the potential to overcome the major limitations of traditional replacement valves; however, leaflet retraction has led to the failure of TEHVs in preclinical studies. Sequentially varying growth factors over time has been utilized to promote maturation of engineered tissues and may be effective in reducing tissue retraction, yet it is difficult to predict the effects of such treatments due to complex interactions between the cells and the extracellular matrix (ECM), biochemical environment, and mechanical stimuli. We hypothesize that sequential treatments of fibroblast growth factor 2 (FGF-2) and transforming growth factor beta 1 (TGF-ß1) can be used to minimize cell-generated tissue retraction by decreasing active cell contractile forces exerted on the ECM and by inducing the cells to increase the ECM stiffness. Using a custom culturing and monitoring system for 3D tissue constructs, we designed and tested various TGF-ß1 and FGF-2 based growth factor treatments, and successfully reduced tissue retraction by 85% and increased the ECM elastic modulus by 260% compared to non-growth factor treated controls, without significantly increasing the contractile force. We also developed and verified a mathematical model to predict the effects of various temporal variations in growth factor treatments and analyzed relationships between tissue properties, the contractile forces, and retraction. These findings improve our understanding of growth factor-induced cell-ECM biomechanical interactions, which can inform the design of next generation TEHVs with reduced retraction. The mathematical models could also potentially be applied toward fast screening and optimizing growth factors for use in the treatment of diseases including fibrosis.

Evaluating the remediation potential of MgFe2O4-montmorillonite and its co-application with biochar on heavy metal-contaminated soils.

In this work, a novel and efficient magnesium ferrite-modified montmorillonite (MgFe2O4-MMT) compound was prepared. MgFe2O4-MMT and biochar were mixed at 0:10, 1:9, 3:7, 4:6, and 10:0 w/w combinations and were used for heavy metal immobilization in soil polluted with multiple heavy metals. MgFe2O4-MMT can significantly increase soil alkalinity, and it exhibited the most optimal effect in immobilization of heavy metals in soil. The amounts of Cd, Pb, Cu, and Zn that were extracted by the toxicity characteristic leaching procedure (TCLP) decreased by 58.4%, 50.3%, 42.9%, and 24.7%, respectively. MgFe2O4-MMT can immobilize heavy metals through electrostatic interactions and cation exchange processes. Although, the immobilization of potentially toxic elements by MgFe2O4-MMT and biochar was inferior to that by MgFe2O4-MMT. The combined application of MgFe2O4-MMT and biochar dramatically increased the diversity and richness of the soil bacterial community. The Chao1 index for M3B7 treatment group was 1.7 and 1.2 times higher than that for the control and MgFe2O4-MMT treatment groups, respectively. The combination of biochar and MgFe2O4-MMT might be a cost-effective and ecological remediation approach for mild Pb and Cd contamination.

Effect of correlation between traction forces on tensional homeostasis in clusters of endothelial cells and fibroblasts.

The ability of cells to maintain a constant level of cytoskeletal tension in response to external and internal disturbances is referred to as tensional homeostasis. It is essential for the normal physiological function of cells and tissues, and for protection against disease progression, including atherosclerosis and cancer. In previous studies, we defined tensional homeostasis as the ability of cells to maintain a consistent level of cytoskeletal tension with low temporal fluctuations. In those studies, we measured temporal fluctuations of cell-substrate traction forces in clusters of endothelial cells and of fibroblasts. We observed those temporal fluctuations to decrease with increasing cluster size in endothelial cells, but not in fibroblasts. We quantified temporal fluctuation, and thus homeostasis, through the coefficient of variation (CV) of the traction field; the lower the value of CV, the closer the cell is to the state of tensional homeostasis. This metric depends on correlation between individual traction forces. In this study, we analyzed the contribution of correlation between traction forces on traction field CV in clusters of endothelial cells and fibroblasts using experimental data that we had obtained previously. Results of our analysis showed that positive correlation between traction forces was detrimental to homeostasis, and that it was cell type-dependent.

Impervious Surfaces Alter Soil Bacterial Communities in Urban Areas: A Case Study in Beijing, China.

The rapid expansion of urbanization has caused land cover change, especially the increasing area of impervious surfaces. Such alterations have significant effects on the soil ecosystem by impeding the exchange of gasses, water, and materials between soil and the atmosphere. It is unclear whether impervious surfaces have any effects on soil bacterial diversity and community composition. In the present study, we conducted an investigation of bacterial communities across five typical land cover types, including impervious surfaces (concrete), permeable pavement (bricks with round holes), shrub coverage (Buxus megistophylla Levl.), lawns (Festuca elata Keng ex E. Alexeev), and roadside trees (Sophora japonica Linn.) in Beijing, to explore the response of bacteria to impervious surfaces. The soil bacterial communities were addressed by high-throughput sequencing of the bacterial 16S rRNA gene. We found that Proteobacteria, Actinobacteria, Acidobacteria, Bacteroidetes, Chloroflexi, and Firmicutes were the predominant phyla in urban soils. Soil from impervious surfaces presented a lower bacterial diversity, and differed greatly from other types of land cover. Soil bacterial diversity was predominantly affected by Zn, dissolved organic carbon (DOC), and soil moisture content (SMC). The composition of the bacterial community was similar under shrub coverage, roadside trees, and lawns, but different from beneath impervious surfaces and permeable pavement. Variance partitioning analysis showed that edaphic properties contributed to 12% of the bacterial community variation, heavy metal pollution explained 3.6% of the variation, and interaction between the two explained 33% of the variance. Together, our data indicate that impervious surfaces induced changes in bacterial community composition and decrease of bacterial diversity. Interactions between edaphic properties and heavy metals were here found to change the composition of the bacterial community and diversity across areas with different types of land cover, and soil properties play a more important role than heavy metals.

The relationships between urban parks, residents' physical activity, and mental health benefits: A case study from Beijing, China.

The role of urban parks in improving public health has been analyzed in the context of urban design in developed countries, but has seldom been considered in developing countries such as China. Previous studies have found positive correlations between parks and residents' physical activity and mental health status. In this study, we conducted a questionnaire survey to investigate respondents' physical activity status and its relationship with urban parks. The impact of different activities engaged in during park use on positive mental health was examined. The average physical activity level of the sample was 92.7 min of moderate to vigorous physical activity per day. Park users were more active in all forms of physical activity, except transport walking, than non-users. The presence of a park within 500 m from home and park use were significantly associated with total physical activity. Physical activity in parks significantly restored visitors' moods and energy levels, and interaction with nature brought mental health benefits in terms of relaxation and self-perceived confidence. Overall, this study found a positive correlation of urban parks with public physical activity and positive mental health benefits. However, further research is needed to improve the understanding of this relationship in the context of China.